Another monoclonal antibodies were purchased out-of Pharmingen (San diego, Calif
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Another monoclonal antibodies were purchased out-of Pharmingen (San diego, Calif Cell staining and you will flow cytometry. Single-cell suspensions were prepared from different lymphoid organs and incubated for 10 min at 10 6 cells/20 ?l on ice in staining buffer (phosphate-buffered saline [PBS] containing 0.5% …
